WIO E5 MIni stopped to work without ST-LINK programmer

I programmed the WIO 5E mini, which works only if attached to the ST-LINK V2 programmer. When I detach it and connect a USB cable to it, it doesn’t work. It happened after the last programming. It doesn’t display any debug messages, but I didn’t disable longer messages. Who does know what happened? The WIO worked former disconnected from the programmer and displayed debug messages.